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Flow chart outlining a strategy for screening T-DNA lines for the presence of chromosomal translocations. In this example the T-DNA insertion is present in the Columbia ecotype (Col). ‘Abnormal pollen’ refers to the situation where a heterozygous plant produces ca. 50% non-viable pollen. Co-segregation of the 5′ and 3′ T-DNA junctions can only be performed when both junctions are detectable by PCR, which may not be the case with all T-DNA lines.
