Table 1.
Strains Used in This Study
| Strain designationa | Genotype | Description of gene product | Referenceb |
|---|---|---|---|
| X1-001 | Parent strain | Strain 10403S; serotype 1/2a | Bishop and Hinrichs (1987) |
| K4-006 | ΔinlA | Internalin A, required for internalization into selected host cells | Bakardjiev et al. (2004) |
| H6-199 | ΔflaA | Flagellum structural protein | O'Neil and Marquis (2006) |
| B4-007 | ΔlisRKc | LisRK, contributes to log-phase acid resistance | This study |
| B2-078 | ΔagrA | Response regulator (RR), contributes to protein secretion | This study |
| B2-080 | ΔresD | RR, contributes to virulence gene repression in presence of select carbohydrates | This study |
| B2-086 | Δlmo1022 | Putative RR, no known role | This study |
| B2-096 | Δlmo1060 | Putative RR, no known role | This study |
| C5-017 | Δlmo2010 | Putative RR, no known role | This study |
| C5-019 | Δlmo2583 | Putative RR, no known role | This study |
| B2-100 | ΔphoP | RR, similar to Bacillus subtilis PhoP | This study |
| C5-041 | ΔvirR | Novel RR involved in invasion of Caco-2 cells | This study |
| C5-036 | Δlmo1507 | Putative RR, no known role | This study |
| C5-033 | ΔdegU | RR, regulates expression of motility genes | This study |
| B2-104 | ΔkdpE | RR, contributes to growth at high osmoloarity and low temperature | This study |
| B2-105 | ΔcheY | RR, contributes to chemotaxis | This study |
| B2-102 | ΔcesR | RR, contributes to ethanol and β-lactam tolerance | This study |
| A1-254 | ΔsigB | Alternative sigma factor σB, regulates general stress response | Wiedmann et al. (1998) |
| B2-046 | ΔprfA | PrfA, regulation of virulence genes | Cheng and Portnoy (2003) |
| B2-068 | ΔsigBΔprfA | McGann et al. (2007b) | |
| I1-001 | ΔinlAΔflaA | Marquis Lab |
a
All strain designations carry the prefix FSL.
b
References where a given mutant was previously described.
c
This mutant includes an internal deletion of the lisR gene, which also removed a portion of the ribosome binding site of lisK sensory kinase gene.
RR, response regulator.