Table 2.
|
|
Caco-2 invasion efficiency (calculated as [CFU recovered/CFU infected] × 100) (SD) fora |
||||
---|---|---|---|---|---|---|
Growth temperature | Temperature of PBS hold (2.5 h) | 10403S | ΔcheY | ΔdegU | ΔflaA | ΔinlA |
30°C | None | 0.67 (0.17)A | 0.018 (0.007)B,* | 0.015 (0.008)B,* | 0.014 (0.010)B,* | 0.024 (0.010)A,* |
30°C | 30°C | 0.64 (0.19)A | 0.013 (0.003)B,* | 0.010 (0.005)B,* | 0.010 (0.006)B,* | 0.023 (0.007)A,* |
30°C | 37°C | 0.36 (0.10)A | 0.006 (0.002)B,* | 0.007 (0.002)B,* | 0.007 (0.004)B,* | 0.014 (0.005)A,* |
37°C | None | 0.14 (0.03)B | 0.14 (0.06)A | 0.20 (0.05)A | 0.14 (0.06)A | 0.0008 (0.0003)B,* |
37°C | 37°C | 0.11 (0.04)B | 0.12 (0.05)A | 0.14 (0.10)A | 0.09 (0.06)A | 0.0007 (0.0007)B,* |
37°C | 30°C | 0.11 (0.03)B | 0.13 (0.05)A | 0.17 (0.08)A | 0.14 (0.07)A | 0.0007 (0.0005)B,* |
Within a given column values with identical letters (A or B) are not significantly different (p ≥ 0.05; post hoc Tukey HSD test). Within a given row, invasion efficiencies that are lower for a given mutant than the parent strain exposed to the same condition (p ≤ 0.05; post hoc Dunnett's test) are marked with an asterisk (*). Data represent the mean and standard deviation of three biological replicates.
PBS, phosphate-buffered saline; SD, standard deviation; ANOVA, analysis of variance; HSD, honestly significantly different.