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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Jan 26;186(5):2959–2969. doi: 10.4049/jimmunol.1000112

FIGURE 7. Triad3A alters 2DL4 turnover and vesicle-mediated internalization is required for cytokine production.

FIGURE 7

A, 2DL4 internalization was not affected in KHYG-1 cells co-transduced with Triad3A. KHYG-1 cells transduced with FLAG-2DL4 ± Triad3A were assayed for 2DL4 internalization as in Materials and Methods. Results are mean ±S.D. of 8 experiments. B, 2DL4 turnover was suppressed in KHYG-1 cells co-transduced with Triad3A. KHYG-1 cells expressing FLAG-2DL4 ± Triad3A were assayed for 2DL4 turnover as in Materials and Methods. Results are mean ±S.D. from 3 experiments. C, Dominant negative (DN) dynamin blocks 2DL4-mediated IL-8 production in 293T cells. 293T cells were not transfected (NT) or transfected with FLAG-2DL4 ± DN-dynamin and tested 48 hr later for IL-8 production by ELISA. D, Dynasore inhibits 2DL4-mediated IL-8 production in 293T cells. 293T cells were transfected with FLAG-2DL4 and cultured with indicated concentrations of dynasore for 48 h and tested for IL-8 secretion by ELISA. E, 2DL4-mediated IL-8 production in KHYG-1 cells was suppressed by dynasore. KHYG-1 cells pretreated with 40 μM dynasore for 30 min were stimulated with either plate-bound anti-FLAG mAb (M2) or anti-NKp44 (3.43.13) and tested 24 h later for IL-8 secretion by ELISA.