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. 2011 Feb 16;36(6):1260–1274. doi: 10.1038/npp.2011.12

Figure 5.

Figure 5

The mGluR2/3 and NMDA receptors are not co-localized in the PFC. Both mGluR2/3 (a) 96 kD) and NR2B (b) 180 kD) were immunoprecipitated with anti-mGluR2/3 and anti-NR2B antibodies, respectively, and were confirmed by western bolt (a, b; left panel). The immunoprecipitated mGluR2/3 proteins did not express NR2B (a; right panel) and immunoprecipitated NR2B proteins did not express mGluR 2/3 (b; right panel), confirming that co-localization is not found between these two receptors. The inputs represented the mGluR2/3 (a; left panel) or NR2B (b; left panel) signal. Wash denotes the negative control, which was treated with the wash-in supernatant of IP-mGluR2/3 (a; left panel) or IP-NR2B (b; left panel) after the immunoprecipitated samples were centrifuged.