Table 1.

Expression of neural crest, Schwann cell, smooth muscle, and neuronal markers in cis-Nf1: p53 tumor cell lines. Up to 41 tumor cell lines isolated from 10 cis-Nf1:p53 sarcomas were processed for immunocytochemistry. Immunostaining patterns for SMA, S100, GFAP, and c-neu were confirmed by immunoblot analysis (20). Four control cell lines were isolated from two Nf1^+/+: p53^−/− sarcomas; all four were positive for smooth muscle markers SMA and calponin but negative for glial, neuronal, and neural crest markers. +++, Strong immunostaining; ++, moderate immunostaining; +, weak immunostaining; −, no detectable immunostaining.

Marker	+++	++	+	−
p75	0/6	2/6	4/6	0/6
c-neu	2/10	8/10	0/10	0/10
GAP-43	0/6	3/6	3/6	0/6
S100	0/36	15/36	20/36	1/36
GFAP	0/21	0/21	13/21	8/21
SMA	3/41	27/41	11/41	0/41
Calponin	4/35	23/35	8/35	0/35
Neurofilament	1/34	4/34	7/34	22/34