Figure 1.
Expression of endothelin system components in the GCs of a human pre-ovulatory follicle. (A) mRNA expression profiles of endothelin components in GCs obtained from a pre-ovulatory follicle. After 40 cycles of PCR amplifications, the PCR products were separated by electrophoresis on 1.2% agarose gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. The real-time amplification profiles (B) and melting curves (C) of endothelin component mRNA. Purple, EDN1; yellow, EDN2; red, EDN3; yellowish green, ETA; green, ETB; sky blue, ECE1; blue, ECE2; pink, GAPDH. (D) EDN3 mRNA expression profiles in GC of eight different patients and BE2 cell line that constitutively expresses EDN3 mRNA. β-Actin was used as an internal control.