Figure 4.
Comparison of endothelin system component expression between human pre-ovulatory follicles with immature oocyte versus mature oocytes. Equal amounts of mRNAs isolated from human immature or mature CCs that were isolated from the same ovary were reverse transcribed and amplified using real-time PCR. The expression level was calculated from CT values, and the mRNA ratio was determined relative to that of mature CC (n = 7). Data are the mean ± SEM. Note that there was no difference in the mRNA level for any of the endothelin components.