. 2011 Mar 15;26(5):1171–1180. doi: 10.1093/humrep/der066

Table I.

Primer sequences for real-time PCR analyzes.

Gene	GeneBank accession number	Oligonucleotide sequence	Exon	Size (bp)
EDN1	BC009720	F- TCGTCCCTGATGGATAAAGA	exon2 ∼ exon3	205
EDN1	BC009720	R- GGCAAAAATTCCAGCACTTC	exon2 ∼ exon3	205
EDN2	BC034393	F- TCCTGAACAGACAGCTCCTT	exon2 ∼ exon8	242
EDN2	BC034393	R- GAAATGTCCCTCAGCCTTTG	exon2 ∼ exon8	242
EDN3	BC053866	F- CTATTGCCACCTGGACATCA	exon2 ∼ exon4	231
EDN3	BC053866	R- GCCGTCCTTGAATTACTGCT	exon2 ∼ exon4	231
ET_A	BC022511	F-AAGGAATGGCAGCTTGAGAA	exon6 ∼ exon9	300
ET_A	BC022511	R-CAGAGGCATGACTGGAAACA	exon6 ∼ exon9	300
ET_B	BC014472	F-TTTGCCTGGTCCTTGTCTTT	exon6 ∼ exon8	286
ET_B	BC014472	R-AAGCACGACTGCTTTTCCTC	exon6 ∼ exon8	286
ECE1	BC117256	F-AGCTCTTCTTCCTGGGCTTT	exon20 ∼ exon21	203
ECE1	BC117256	R-TTACCAGACTTCGCACTTGT	exon20 ∼ exon21	203
ECE2	BC069005	F-CTTCCCCAGTGCTTCTTTTG	exon2 ∼ exon3	207
ECE2	BC069005	R-GCATAGTGTCTGGTCCGAAA	exon2 ∼ exon3	207
β-Actin	NM_001101	F-AGGCCAACCGCGAGAAGATGACC	exon1	311
β-Actin	NM_001101	R-GAAGTCCAGGGCGACGTAGCAC	exon1	311
GAPDH	NM_002046	F-ACCACAGTCCATGCCATCAC	exon5 ∼ exon6	451
GAPDH	NM_002046	R-TCCACCACCCTGTTGCTGTA	exon5 ∼ exon6	451

GADPH, glyceraldehyde-3-phosphate dehydrogenase; EDN, endothelin; ECE, endothelin-converting enzyme; ET, endothelin receptor.