Fig. 4.

No effect of Hsp90 inhibitors on SMC3-induced c-IAP1 degradation and TNF-α secretion. a H23 cells were pre-treated with 17AAG for 10 h followed by SMC3 for the indicated times. c-IAP1 and c-IAP2 were detected by Western blot. β-tubulin was detected as an input control. b H23 or HepG2 cells were pretreated with 17AAG (50 nM) for 10 h followed by SMC3 treatment (50 nM) for the indicated times. The concentration of TNF in cell culture media was measured by ELISA. c H23 cells were pretreated with CCT018159 (10 μM) or rifabutin (10 μM) for 10 h followed by SMC3 treatment (50 nM) for the indicated times. The concentration of TNF in cell culture media was measured by ELISA