Fig. 5.

Inhibiting Hsp90 enhances SMC-3 induced apoptosis. H23 (a) or HepG2 (b) cells were pretreated with 17AAG (50 nM) for 10 h or left untreated and followed by SMC3 treatment (25 nM for H23 and 50 nM for HepG2 cells) for the indicated time periods. Caspase-8, PARP, and activated caspase-3 were detected by Western blot. β-tubulin was detected as an input control. c H23 cells were pretreated with 17AAG (50 nM) for 10 h or left untreated and followed by SMC3 treatment (25 nM) for 30 h. The cells were stained with propidium iodide (100 μg/ml) for 30 min and analyzed by flow cytometry