Table 1.
Efficiency of the InSITE genetic swap procedure. At least one line, chosen at random, for each of the six genetic donor elements was tested for the ability to excise and re-integrate into an attP-containing recipient site. Three different enhancer trap target lines were tested. Rates of eyFLP-resistant white+ flies and true integration events, as assayed by PCR, are shown.
| Recipient | Donor (Line #) | # of crosses | # of crosses with w+ flies | % of crosses with w+ flies | # tested by PCR | # of true integrations | % of true integrations | Recovered swap |
|---|---|---|---|---|---|---|---|---|
| PBac{IT.GAL4.w−}6.1 | P{ID.VP16AD}D33.1 | 26 | 9 | 34.6 | 9 | 7 | 77.8 | yes |
| PBac{IT.GAL4.w−}3.1 | P{ID.VP16AD}D33.1 | 39 | 9 | 23.1 | 8 | 1 | 12.5 | yes |
| PBac{IT.GAL4.w−}6.1 | P{ID.VP16AD}D37.1 | 34 | 8 | 23.5 | no data | no data | no data | yes |
| PBac{IT.GAL4.w−}3.1 | P{ID.VP16AD}D37.1 | 30 | 7 | 23.3 | 7 | 0 | 0.0 | no |
| PBac{IT.GAL4.w−}6.1 | P{ID.GAL4DBD}F32.1 | 30 | 5 | 16.7 | 5 | 3 | 60.0 | yes |
| PBac{IT.GAL4.w−}3.1 | P{ID.GAL4DBD}F32.1 | 30 | 10 | 33.3 | 9 | 4 | 44.4 | yes |
| PBac{IT.GAL4.w−}6.1 | P{ID.GAL80}E17.1 | 29 | 10 | 34.5 | no data | no data | no data | yes |
| PBac{IT.GAL4.w−}3.1 | P{ID.GAL80}E17.1 | 30 | 17 | 56.7 | no data | no data | no data | yes |
| PBac{IT.GAL4.w−}6.1 | P{ID.QF}Q10B | 20 | 4 | 20.0 | 4 | 3 | 75.0 | yes |
| PBac{IT.GAL4.w−}3.1 | P{ID.QF}Q12A | 18 | 5 | 27.8 | 5 | 3 | 60.0 | yes |
| PBac{IT.GAL4.w−}5.1 | P{ID.LexA}L34.2L | 19 | 6 | 31.6 | 6 | 6 | 100.0 | yes |
| PBac{IT.GAL4.w−}5.1 | P{ID.GAL4AD}G12.1 | 19 | 5 | 26.3 | 5 | 3 | 60.0 | yes |
| Total | - | 324 | 95 | 29.3 | 58 | 30 | 51.7 | 12 of 13 |