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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Nat Methods. 2011 Mar;8(3):231–237. doi: 10.1038/nmeth.1561

Table 1.

Efficiency of the InSITE genetic swap procedure. At least one line, chosen at random, for each of the six genetic donor elements was tested for the ability to excise and re-integrate into an attP-containing recipient site. Three different enhancer trap target lines were tested. Rates of eyFLP-resistant white+ flies and true integration events, as assayed by PCR, are shown.

Recipient Donor (Line #) # of crosses # of crosses with w+ flies % of crosses with w+ flies # tested by PCR # of true integrations % of true integrations Recovered swap
PBac{IT.GAL4.w−}6.1 P{ID.VP16AD}D33.1 26 9 34.6 9 7 77.8 yes
PBac{IT.GAL4.w−}3.1 P{ID.VP16AD}D33.1 39 9 23.1 8 1 12.5 yes
PBac{IT.GAL4.w−}6.1 P{ID.VP16AD}D37.1 34 8 23.5 no data no data no data yes
PBac{IT.GAL4.w−}3.1 P{ID.VP16AD}D37.1 30 7 23.3 7 0 0.0 no
PBac{IT.GAL4.w−}6.1 P{ID.GAL4DBD}F32.1 30 5 16.7 5 3 60.0 yes
PBac{IT.GAL4.w−}3.1 P{ID.GAL4DBD}F32.1 30 10 33.3 9 4 44.4 yes
PBac{IT.GAL4.w−}6.1 P{ID.GAL80}E17.1 29 10 34.5 no data no data no data yes
PBac{IT.GAL4.w−}3.1 P{ID.GAL80}E17.1 30 17 56.7 no data no data no data yes
PBac{IT.GAL4.w−}6.1 P{ID.QF}Q10B 20 4 20.0 4 3 75.0 yes
PBac{IT.GAL4.w−}3.1 P{ID.QF}Q12A 18 5 27.8 5 3 60.0 yes
PBac{IT.GAL4.w−}5.1 P{ID.LexA}L34.2L 19 6 31.6 6 6 100.0 yes
PBac{IT.GAL4.w−}5.1 P{ID.GAL4AD}G12.1 19 5 26.3 5 3 60.0 yes
Total - 324 95 29.3 58 30 51.7 12 of 13
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