Fig. 3. The observed bile-induced phospholipid changes in V. cholerae occur at very low bile concentrations and within 15m of bile exposure.

A. The migratory shift of phospholipids occurs over a range of bile concentrations. Bacteria were grown to mid-log phase in media with the indicated bile concentrations and ³²P_i prior to lipid extraction. Densitometry data can be found in Table S3.

B. The altered phospholipid profile is observed just 15m after bile exposure and is sustained at least 2 hours when constitutively grown in bile. Bacteria were grown to an A₆₀₀ of ~ 0.5 (0m) prior to pelleting of the bacteria and resuspension in LB supplemented with 0.4% bile containing ³²P_i. Samples were taken at the indicated times post bile exposure. Densitometry data can be found in Table S3.