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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 7;79(3):716–728. doi: 10.1111/j.1365-2958.2010.07476.x

Fig. 5. Mass spectrometric analysis of CL extracted from V. cholerae grown in the presence and absence of bile.

Fig. 5

A. Negative-ion ESI-MS of [M-2H]2− ions of V. cholerae CL isolated from cultures grown in the presence and absence of bile. Selected peaks (*) found only in the presence of bile were subsequently analyzed by MS/MS.

B. Negative-ion MS/MS analysis of the [M-2H]2− ions at m/z 712.525 and 725.546 detected only in the presence of bile. The product-ion spectra show that these CL species contain longer, polyunsaturated fatty acyl chains (18:2, 20:4 and 22:5).