Table 1.

Antibodies used for the immunohistochemical examination

Antibody	Clone	Source	Dilution	Antigen retrieval	Interpretation
CD44 extracellular domain (aminoterminal end)	Polyclonal, Ab64929	Abcam, Cambridge, MA	1/50	Not applied	Sinicrope's method*
Zo-1	Polyclonal, 61-7300	Zymed, South San Francisco, CA	1/100	Protease treatment (15 min, 37°C)	Linear or dot-like
Occludin	Polyclonal, 71-1500	Zymed	1/100	Protease treatment (15 min, 37°C)	Linear or dot-like
MMP-7	Monoclonal, 141-7B2	Daiichi Fine Chemical, Takaoka, Japan	1/200	Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])	Sinicrope's method*
Pancytokeratin	Monoclonal, MNF116	Dakocytomation, Glostrup, Denmark	1/50	Microwave treatment for 15 min (Dako target retrieval solution [pH 9.0])	For evaluation of tumor budding
beta-Catenin	Monoclonal, 14/beta-catenin	BD Transduction laboratories, Lexington, KY	1/200	Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])	Nuclear, more than 20% judged as positive; membranous and cytoplasmic, Sinicrope's method*
Laminin-5γ2	Monoclonal, 4G1	Dakocytomation	1/50	Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])	Sinicrope's method*
Sialyl Lewis X	Monoclonal, FH6	Ohtsuka Pharmaceutical, Tokushima, Japan	1/2000	Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0])	Sinicrope's method*

• Sinicrope's method is described in Reference [27].