Table 1.
Antibody | Clone | Source | Dilution | Antigen retrieval | Interpretation |
---|---|---|---|---|---|
CD44 extracellular domain (aminoterminal end) | Polyclonal, Ab64929 | Abcam, Cambridge, MA |
1/50 | Not applied | Sinicrope's method* |
Zo-1 | Polyclonal, 61-7300 | Zymed, South San Francisco, CA | 1/100 | Protease treatment (15 min, 37°C) | Linear or dot-like |
Occludin | Polyclonal, 71-1500 | Zymed | 1/100 | Protease treatment (15 min, 37°C) | Linear or dot-like |
MMP-7 | Monoclonal, 141-7B2 | Daiichi Fine Chemical, Takaoka, Japan | 1/200 | Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0]) | Sinicrope's method* |
Pancytokeratin | Monoclonal, MNF116 | Dakocytomation, Glostrup, Denmark | 1/50 | Microwave treatment for 15 min (Dako target retrieval solution [pH 9.0]) | For evaluation of tumor budding |
beta-Catenin | Monoclonal, 14/beta-catenin | BD Transduction laboratories, Lexington, KY | 1/200 | Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0]) | Nuclear, more than 20% judged as positive; membranous and cytoplasmic, Sinicrope's method* |
Laminin-5γ2 | Monoclonal, 4G1 | Dakocytomation | 1/50 | Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0]) | Sinicrope's method* |
Sialyl Lewis X | Monoclonal, FH6 | Ohtsuka Pharmaceutical, Tokushima, Japan | 1/2000 | Microwave treatment for 15 min (Dako target retrieval solution [pH 6.0]) | Sinicrope's method* |
• Sinicrope's method is described in Reference [27].