Table 1.
Bacterial strains and plasmids used in this study
| Strains & plasmids | Description | Source or Reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | endA1 gyrA96 (nalR) thi-1 recA1 relA1 lac glnV44 F'[::Tn10 proAB+ lacIq Δ(lacZ) M15] hsdR17 (rK- mK+) | Stratagene |
| R. eutropha H16 | Wild type (PHB+) | DSMZ 428 |
| Plasmids | ||
| pBHR68 | Ampr, pBluescript SK-, harboring PHB synthesis operon from R. eutropha (phaCAB operon), used to produce PHB granules | 16 |
| pP-CAB | Ampr , pBHR68:phaP under control of phaCAB promoter | This study |
| pFos-P-CAB | Ampr , pBHR68: bFos-phaP under control of phaCAB promoter | This study |
| pJun-P-CAB | Ampr , pBHR68: bJun-phaP under control of phaCAB promoter | This study |
| pATF2-P-CAB | Ampr , pBHR68: bATF2-phaP under control of phaCAB promoter | This study |
| pFos | Ampr , pBHR68Δ(phaCAB):bFos under control of phaCAB promoter | This study |
| pFos-P | Ampr , pBHR68Δ(phaCAB): bFos-phaP under control of phaCAB promoter | This study |
| pACYC184 | Cmr, Tetr, cloning vector | Fermentas |
| pOZ | Cmr, pACYC184:lacZ under control of phaP promoter | This study |
| pDBD-Jun-Z | Cmr, pOZ:DBD-bJun under control of phaCAB promoter | This study |
| pDBD-Z | Cmr, pOZ:DBD under control of phaCAB promoter | This study |
| pPI-LacZ | Ampr, pTWIN2:phaP-intein-lacZ | 17 |