Figure 6.

Effects of adrenomedullin (AM) on TGF-β. immunohistochemical analysis of lung sections obtained from mice treated with BLM revealed positive staining for TGF-β at 14 (B) and 21 (F) days after treatments. In BLM mice treated with AM, positive staining for TGF-β was significantly reduced at 14 (C) and 21 (G) days. Densitometry analysis (D and H) of immunohistochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for TGF-β was assessed. The assay was carried out by using AxioVision on a personal computer. The figure is representative of at least three experiments performed on different experimental days. Data are expressed as % of total tissue area and are mean ± standard deviation from n = 10 mice for each group. *P < 0.01 vs sham, °P < 0.01 vs bleomycin+ vehicle.