Table 2.
Short term vapor storage of vitrified embryos on different carriers to simulate transport conditions
| Cleavage Stage Vitrification | ||||||
|---|---|---|---|---|---|---|
| Carrier |
Cryoloop (n = 40) |
HSV Straw (n = 35) |
Cryotip (n = 40) |
|||
| Storage Condition | LN2 | Vapor | LN2 | Vapor | LN2 | Vapor |
| Survival (%) | 100 | 100 | 100 | 100 | 100 | 100 |
| Development to blastocyst after 48 hours (%) | 100 | 100 | 100 | 93 | 100 | 100 |
| Total blastomeresa (mean ± SD) | 82.21 ± 13.28 | 89.18 ± 18.52 | 87.20 ± 10.67 | 88.5 ± 9.55 | 81.10 ± 14.09 | 75.53 ± 17.62 |
| Blastocyst Stage Vitrification | ||||||
| Carrier |
Cryoloop (n = 41) |
HSV straw (n = 40) |
Cryotip (n = 35) |
|||
| Storage Condition | LN2 | Vapor | LN2 | Vapor | LN2 | Vapor |
| Survival (%) | 100 | 100 | 100 | 100 | 100 | 100 |
| Re-expansion (%) | 90 | 81 | 95 | 85 | 80 | 85 |
| Total blastomeresa (mean ± SD) | 108 ± 18 | 96 ± 19 | 96 ± 20 | 90 ± 20 | 96 ± 4 | 86 ± 19 |
a Total cell count at termination of experiment for both Vit-CL and Vit-BL on day 5
No significant difference in survival, development, re-expansion or cell number after short term vapor storage as compared to liquid nitrogen