Table 1.

Effect of substrate concentration on the yield of 9-HPOD from linoleic acid catalyzed by Nb-9-LOX-yeast protein extracts prepared from 1 ml of cell culture in 20 min.

Linoleic acid (μmol)	9-HPOD (μmol)	Yield (%)
12	11.8 ± 0.4	98
15	14.4 ± 0.6	96
18	17.9 ± 1.5	99
24	20.2 ± 3.0	84
30	19.7 ± 0.7	66

The amount of 9-HPOD was determined using a standard curve calculated from various known concentrations of 9-HPOD against the UV peak areas which were recorded at 234 nm by LC-MS. Each value is the average and standard error of two replicates.