Table 4.
Hydraulic conductivity (Lp) and ethylene glycol permeability (PEG) of X. laevis oocytes expressing wild-type (WT) DrAqp3b, two DrAqp3b mutants or HsAQP3, in hypertonic ethylene glycol solution
| Lp (μm/min/atm) | PEG (x 10-3 cm/min) | |
|---|---|---|
| Water | 0.36 ± 0.02 a | 0.47 ± 0.03 a |
| DrAqp3b-WT (1 ng) | 2.15 ± 0.14 c | 17.59 ± 0.83 d |
| DrAqp3b-H53A/G54H/T85A (1 ng) | 1.55 ± 0.13 b | 13.06 ± 0.72 c |
| DrAqp3b-T85A (1 ng) | 2.64 ± 0.15 d | 25.49 ± 1.56 e |
| HsAQP3 (1 ng) | 1.31 ± 0.08 b | 10.73 ± 0.59 b |
| DrAqp3b-WT (20 ng) | 2.63 ± 0.07 d | 40.06 ± 1.22 c |
| DrAqp3b-H53A/G54H/T85A (20 ng) | 2.27 ± 0.07 c | 36.58 ± 1.74 c |
| DrAqp3b-T85A (20 ng) | 3.24 ± 0.08 e | 53.91 ± 2.55 d |
| HsAQP3 (20 ng) | 1.96 ± 0.08 b | 22.24 ± 0.95 b |
Values (mean ± SEM) for water- and aquaporin-injected (1 or 20 ng cRNA) oocytes are calculated from the data in Figure 6B and C. For each cRNA dose, data with different superscript are statistically significant (ANOVA, p < 0.05).