Table 1.
Primers used for the qPCR amplifications.
| Insect | Target gene | Primer pair | Primer name | Sequence (5'→3') |
|---|---|---|---|---|
| D. melanogaster | RpS3 | s3-1 | s3-1 F | TCTTTCTTTTCTGCGCACCA |
| s3-1 R | TCGCATTCATTTTGACGTCG | |||
| BarH1 | h1-4 | h1-4 F | CCAGGACGATCCGTTGACA | |
| h1-4 R | GATCTGATCCTCGTCGTCCG | |||
| h1-5 | h1-5 F | TCCAGGTGTGAGCGGTACG | ||
| h1-5 R | ATTAGCACACGCACACAATCG | |||
| BarH2 | h2-4 | h2-4 F | CAGAACCAAATGGAAGCGTCA | |
| h2-4 R | TCGGCCAGCAGTTCCAAG | |||
| h2-7 | h2-7 F | GGAGGAACTGGCCCTGGA | ||
| h2-7 R | GGAGGGTTGAATTCTCTCGGA | |||
| O. nubilalis | RpS3 | s3-1 | s3-1 F | GAGCTACTGGGAGAGAAGG |
| s3-1 R | GATGTTGAAACGCTTCTGGA | |||
| s3-2 | s3-2 F | GCGTTTCAACATCCCTGAACA | ||
| s3-2 R | GGCGACTTTCTCAGCGTACAG | |||
| tpi | t1 | t1 F | GGCGACAAGAATCAAATCAATG | |
| t1 R | AGGACCCTTTTTCAGAGTGTTCAC | |||
| ldh | l1 | l1 F | GAATAAATCGGGCTCGAAGGAC | |
| l1 R | TCACGCGAGACAGCTTCAAA | |||
| cadherin | e26 | e26F | ACGGCAACAACGAGGGTCT | |
| e26R | GAGATGACGTTGCGCGACT | |||
| e28 | e28F | CGAGCCACACAGAAGACGAC | ||
| e28R | TGCTCGCACGGTCTATGATG | |||
The letters F or R at the end of the primer names refer to their respective orientation (F = forward; R = reverse). The qPCR product sizes were 51 base pairs in all reactions except for primer pairs s3-1 and e26 of O. nubilalis, with lengths of 70 and 52 base pairs respectively.