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Kinetic class analysis of DEV UL15. DEV-infected CEF cells were harvested at the indicated time post-infection, followed by mRNA detection by RT-PCR (A) or protein detection by western blot (B). The procedures were done with 100 μg/ml cycloheximide (CHX) or with 300 μg/ml phosphonoacetic acid (PAA) or without metabolic inhibitor as the control. Chicken β-actin mRNA or protein was used as an internal control.
