Table 2.
Primers used in the this study
| Primer name | Primer sequences (5' to 3') | Note |
|---|---|---|
| P15f | ATAGGATCCATGTTCGGGGCAACTTTCG | UL15 forward/BamHIa |
| P15r | GGCAAGCTTTGGTACATATCCTACGTGCGC | UL15 reverse/HindIIIa |
| Psjf | TTGAATTATTCCAGAAGATGA | Splice junction forward |
| Psjr | TTTCTTGCATAAATGAATCG | Splice junction reverse |
| P898f | AATTTGGGCAACTCAATGGTT | Northern probe forward |
| P898r | TCTGCCGTACGTCTCATAGC | Northern probe reverse |
| P15-5 | TGGTGCATGCTCGCAACAGT | UL15 5'-RACE primer |
| P15.5-5 | TGTTCCAACCCGTATATTAC | UL15.5'-RACE primer |
| P15-3 | TTACCACTGCGTGCCTCCCA | UL15 3'-RACE primer |
| Paf | GCGCTCGTTGTTGACA | β-actin forward |
| Par | TCATCCCAGTTGGTGACA | β-actin reverse |
aThe incorporated BamHI and HindIII enzyme sites within P15f and P15r are underlined within primer sequences respectively.