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View full-text article in PMC

. 2011 Mar 30;11:113. doi: 10.1186/1471-2407-11-113

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Copyright ©2011 Zeng et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of huMETCAM/MUC18 expression on a disorganized growth of MDA-MB-231 and MDA-MB-468 cell lines in 3D basement membrane culture assay. The embedded 3D basement membrane culture assay was carried out as described in "Materials and Methods". Panels A and D, in which no antibody was added, show a disorganized growth of both MDA-MB-231 and MDA-MB-468 cells in 3D culture assay, respectively. Panels B and E, in which the control isotype antibody (Chicken IgY) was added, show results similar to Panels A and D. Panels C and F show that the disorganized growth of both MDA-MB-231 and MDA-MB-468 cells in 3D culture assay was destroyed in the presence of 15 μg/ml of anti-huMETCAM/MUC18 antibody, respectively.