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. 2011 Mar 30;11:113. doi: 10.1186/1471-2407-11-113

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Copyright ©2011 Zeng et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of huMETCAM/MUC18 expression on MCF7 cells in the formation of anchorage-independent colonies. Abilities of MCF7 clones to form anchorage-independent colonies were determined by the soft agar colony formation assay as described in "Materials and Methods". Quantitative results of the formation of anchorage-independent colonies by the pooled 2D clone, which was transfected with the huMETCAM/MUC18 cDNA gene, and the pooled 3D clone, which was transfected with the empty vector, are shown. The mean values and standard deviations from the colony numbers in at least four wells are shown.