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Lack of IL-6 signaling in canine OSA. The canine OSA cell line OSA16 was serum starved for two hours then stimulated with rcIL6 (30 ng/mL) for 0, 5, 10, or 30 minutes. Protein lysates were generated and separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), total STAT3, pJAK2 (Y1007/1008), and total JAK2 was performed.
