(A) ILeak conducted by the U-type channel is partially blocked
by Gd3+. Representative ILeak (A1) and
ILeak - voltage (I–V) relations (A2) of naïve
control, control dsRNA/siRNA, and U-type dsRNA/siRNA 1&2 in saline
with or without 10 µM Gd3+. (B3) Average
normalized ILeak in 10 µM Gd3+ over
saline at -160 mV, −110 mV, and −60 mV for naïve
control (n = 4), control dsRNA
(n = 4), U-type dsRNA (n = 3),
control siRNA (n = 4), U-type siRNA 1
(n = 4), and U-type siRNA 2
(n = 4). The dashed line represents the current
activity in saline. 10 µM Gd3+ reduced
significantly the ILeak in the naïve control and control
dsRNA/siRNA groups, but not in U-type dsRNA/siRNA groups. Data are
presented as mean ± SEM. Significant difference (P<0.05)
between Na+ free saline current normalized to saline is
denoted by *. (B) 10 µM Gd3+ hyperpolarized
the membrane potential. (B1) Representative records of the membrane
potentials of RPeD1 from naïve control, control dsRNA/siRNA, and
U-type dsRNA/siRNA group. 1 & 2 indicate perfusion with and without
10 µM Gd3+, respectively. (B2) Average difference
of the membrane potential by 10 µM Gd3+ in
naïve control (n = 4), control dsRNA
(n = 3), U-type dsRNA (n = 4),
control siRNA (n = 3), U-type siRNA 1
(n = 4), U-type siRNA 2
(n = 3) groups. * and † indicate the
significant difference (P<0.05) between naïve control and U-type
RNAi treatment and between control RNAi and U-type RNAi treatment,
respectively.