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. 2011 Apr 19;6(4):e18481. doi: 10.1371/journal.pone.0018481

Figure 2. Effect of psh-HIF-1α on hypoxia-induced Dll4, HIF-1α and VEGF expression in RF/6A cells determined by real-time RT-PCR and Western blotting.

Figure 2

Messenger RNA (A) and protein (B and C) expression of Dll4, HIF-1α and VEGF in RF/6A cells transfected with either psh-HIF-1α or control pDNA. RF/6A cells were exposed to 200 µM CoCl2 for 6 hrs to achieve chemical hypoxia. After 6 hrs exposure to hypoxia, Dll4, HIF-1α and VEGF expression increased in RF/6A cells transfected with control pDNA (*1 P<0.01). While transfected with psh-HIF-1a, the up-regulation of Dll4, HIF-1α and VEGF in RF/6A cells by hypoxia were abrogated (*2 P<0.01).