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. 2011 Apr 19;6(4):e18481. doi: 10.1371/journal.pone.0018481

Figure 5. Analysis of effect of Dll4 on the proliferation of RF/6A cells co-cultured with RPE cells under hypoxia by MTT proliferation assay.

Figure 5

RF/6A cell growth curves from five treatment groups are depicted. In the proliferation assay, hypoxia increased RF/6A cells proliferation in the co-culture system, while the up-regulation of Dll4 in RF/6A cells promoted cell growth to a remarkable extent (*1 P<0.01 vs control pDNA-transfected group under hypoxia). By contrast, cell growth was suppressed significantly by the silencing of Dll4 (*2 P<0.01) and by the total block of Notch signaling in GSI-treated cells (*3 P<0.01). Noticeably, the proliferation of GSI-treated cells was even lower than that of Si-Dll4-transfected cells (*4 P<0.01 vs Si-Dll4-transfected group under hypoxia).