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. 2011 Apr 19;6(4):e18481. doi: 10.1371/journal.pone.0018481

Figure 8. Analysis of effect of Dll4 on invasion of RF/6A cells across RPE monolayer under hypoxia in a contact co-culture system.

Figure 8

Representative photographs of each group are shown. (A) RPE-RF/6A contact co-culture system. (B) GFP-labeled migrated RF/6A cells from each of the following groups: co-cultured cells transfected with control pDNA, Dll4 up-regulation group, Dll4 down-regulation group and GSI treated group. (C) The number of invaded RF/6A cells overexpressing Dll4 was significantly reduced compared with the control, as was the number of invaded cells in the GSI-treated group; however, the Si-Dll4 transfected group showed a noticeable increasing in invasion (# P<0.05, * P<0.01). Bar = 100 µm.