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. 2011 Apr 19;6(4):e18512. doi: 10.1371/journal.pone.0018512

Figure 1. Atx-3 knockout animals are more resistant to heat stress than wild type.

Figure 1

(A) Survival curves at 35°C of wild type (N2) and atx-3 mutants (gk193, tm1689) previously grown at 20°C (p<0,0001). Median survival of N2 and atx-3 mutants is 9 h and 10 h respectively. (B) Survival curves at 35°C of N2 and atx-3 mutantspreviously grown at 25°C (p<0,0001). Median survival of N2 and atx-3 mutants is 12.5 h and 17 h respectively (p<0,0001). Pre-exposure to a non-lethal heat shock of 2 h (C) or 5 h (D) at 33°C further enhances the thermoresistance phenotype of atx-3 mutants (p<0,0001). Median survivals are: N2: 9 h, atx-3: 10 h, N2 pre-HS2h: 10 h, atx-3 pre-HS2h: 14 h, N2 pre-HS5h: 13 h, atx-3 pre-HS5h: 15 h. The same trend is observed with animals previously grown at 25°C (p<0,0001) both with the 2 h (E) and 5 h (F) pre-HS. Median survivals are: N2: 12.5 h, atx-3: 17 h, N2 pre-HS2h: 18 h, atx-3 pre-HS2h: 22 h, N2 pre-HS5h: 17 h, atx-3 pre-HS5h: 20 h. One representative experiment is shown (at least three independent replicates were performed).