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. 2011 Apr 19;6(4):e18512. doi: 10.1371/journal.pone.0018512

Figure 7. Atx-3 thermoresistance is dependent on DAF-16 transcription factor and independent on HSF-1.

Figure 7

Wild type animals die more than atx-3 mutants after a 5 h (A) or 9 h (B) exposure to 35°C heat shock. Hsf-1 animals have enhanced sensitivity to this temperature at both timepoints, as expected. Double atx-3; hsf-1 mutants still display enhanced thermoresistance at 5 h timepoint but behave as single hsf-1 mutants in the 9 h heat shock condition. Atx-3 mutants are more resistant to the exposure to a lethal heat shock at 35°C than wild type animals, but daf-16; atx-3 double mutants are not, both when grown at 20°C (C) or 25°C (D). (E) Restoring DAF-16 levels in a daf-16; atx-3 (daf-16 deletion and atx-3 knockdown; daf-16 rescue strain) background leads to the appearance of the thermotolerance phenotype. Increased thermotolerance of atx-3 mutants is still observed when grown on bacteria expressing RNAi empty vector (L4440) (F) and is dependent on hsp-16.2 (G), C12C8.1 (H) and F44E5.5 (I). Median survivals in animals grown in (C) are: N2: 12.5 h, atx-3: 17 h, daf-16: 13 h, daf-16; atx-3: 13 h, daf-2: not determined. Median survivals in (D) are: N2: 17 h, atx-3: 19 h, daf-16: 16 h, daf-16; atx-3: 17 h, daf-2: not determined. Median survivals in (E) are: daf-16 rescue in L4440: 14 h, daf-16 rescue in RNAi(ATX-3): 16 h. Median survivals in (F) are: N2 L4440: 21 h, atx-3 L4440: 23 h. N2 in RNAi(HSP-16.2): 19 h, atx-3 in RNAi(HSP-16.2): 17 h. Median survivals in (G) are: N2 L4440: 14 h, atx-3 L4440: 16 h. N2 in RNAi(C12C8.1): 14 h, atx-3 in RNAi(C12C8.1): 14 h. Median survivals in (H) are: N2 L4440: 13 h, atx-3 L4440: 15 h. N2 in RNAi(F44E5.5): 13 h, atx-3 in RNAi(F44E5.5): 13 h. *p<0.05.