Table 3. Known endo- and exo-hemicellulases predicted from the Fibrobacter succinogenes S85 genome.

CAZy Family	Fisuc Locus	FSU Locusa	CBM Family	Basic Terminal domainb	Signal Peptide	In Silico Prediction	Characterized Activity	Reference
GH2	Fisuc_1788	FSU_2288	–	BTD	Yes	β-galactosidase	–	–
GH2	Fisuc_3049	FSU_0315	–	–	No	β-galactosidase	β-galactosidase	This work
GH3	Fisuc_1751	FSU_2249	–	–	Yes	β-xylosidase	–	–
GH3	Fisuc_1985	FSU_2508	–	–	Yes	β-xylosidase	–	–
GH3	Fisuc_2065	FSU_2592	–	–	Yes	β-xylosidase	cellobiosidase	This work
GH10	Fisuc_0754	FSU_1192	–	–	Yes	xylanase	–	–
GH10	Fisuc_0757	FSU_1195	–	–	Yes	xylanase	–	–
GH10	Fisuc_1791	FSU_2292	CBM6	BTD	Yes	xylanase	–	[32]
GH10	Fisuc_1793	FSU_2293	CBM6	BTD	yes	xylanase	xylanase	[32]
GH10	Fisuc_1794	FSU_2294	CBM6	BTD	Yes	xylanase	xylanase	[32]
GH10	Fisuc_2303	FSU_2851	–	BTD	Yes	endoglucanase	–	–
GH10	Fisuc_2992	FSU_0257	–	–	Yes	cellulase	–	[33]
GH11	Fisuc_0362	FSU_0777	–	BTD	Yes	xylanase	xylanase	[34]
GH11	Fisuc_2201	FSU_2741	–	BTD	Yes	xylanase	xylanase	[22]
GH11	Fisuc_2442	FSU_3006	–	–	Yes	xylanase	xylanase	[22]
GH18	Fisuc_1530	FSU_2012	–	–	Yes	chitinase	cellulose binding	[35]
GH18	Fisuc_2465	FSU_3030	–	–	Yes	chitinase	–	–
GH26	Fisuc_0727	FSU_1165	CBM35	–	Yes	mannanase	β-mannanase	This work
GH26	Fisuc_0729	FSU_1167	CBM35	–	Yes	mannanase	β-mannanase	This work
GH26	Fisuc_0730	FSU_1168	–	–	Yes	mannanase	β-mannanase	[22]
GH26	Fisuc_1266	FSU_1729	–	–	Yes	mannanase	–	–
GH26	Fisuc_1688	FSU_2181	CBM35	–	Yes	mannanase	–
GH43	Fisuc_1762	FSU_2262	CBM6	BTD	No	β-xylosidase	–	–
GH43	Fisuc_1763	FSU_2263	CBM6	BTD	Yes	β-xylosidase	–	–
GH43	Fisuc_1764	FSU_2264	CBM6	BTD	Yes	β-xylosidase	–	[22]
GH43	Fisuc_1769	FSU_2269	CBM6	BTD	No	β-xylosidase	arabinoxylanase	This work
GH43	Fisuc_1775	FSU_2274	CBM6	BTD	Yes	β-xylosidase	–	–
GH43	Fisuc_1994	FSU_2517	CBM35, CBM61	–	Yes	β-xylosidase	arabinase	This work
GH43	Fisuc_1997	FSU_2520	–	–	No	xylanase	–	–
GH43	Fisuc_1998	FSU_2521	–	–	Yes	β-xylosidase	–	–
GH43	Fisuc_1999	FSU_2522	CBM35	–	No	β-xylosidase	–	–
GH43	Fisuc_2621	FSU_3190	–	–	Yes	β-xylosidase	–	–
GH43	Fisuc_2622	FSU_3191	CBM35	–	Yes	β-xylosidase	–	–
GH43	Fisuc_2623	FSU_3192	CBM35	–	Yes	β-xylosidase	–	–
GH43	Fisuc_2886	FSU_0145	–	–	Yes	β-xylosidase	–	–
GH43	Fisuc_2929	FSU_0192	CBM6	BTD	No	xylanase	–	–

^aFSU locus tags refer to the equivalent ORF call in the F. succinogenes genome sequence project described by GenBank accession: CP002158.

^bBasic Terminal Domain (BTD) is also known as F. succinogenes-specific paralogous module 1 (FPm-1) [46].