Table 4. Known carbohydrate esterases predicted from the Fibrobacter succinogenes S85 genome.

CAZy Family	Fisuc Locus	FSU Locusa	CBM Family	Basic Terminal domainb	Signal Peptide	In Silico Prediction	Characterized Activity	Reference
CE1	Fisuc_1771	FSU_2270	CBM4	BTD	Yes	esterase	esterase	This work
CE1	Fisuc_1568	FSU_2052	–	–	Yes	esterase	–	–
CE1	Fisuc_1569	FSU_2054	–	–	Yes	esterase	–	–
CE1	Fisuc_1768	FSU_2268			Yes	feruloyl esterase	–	–
CE1	Fisuc_1948	FSU_2468	–	–	Yes	esterase	–	–
CE1	Fisuc_2396	–	CBM4	–	Yes	esterase	–	–
CE2	Fisuc_1641	FSU_2130	–	–	Yes	esterase	esterase	This work
CE6	Fisuc_1766	FSU_2266	CBM6	BTD	Yes	acetyl xylan esterase	acetyl xylan esterase	[36]
CE6	Fisuc_1767	FSU_2267	CBM6	BTD	Yes	acetyl xylan esterase	acetyl xylan esterase	[36], [37]
CE6	Fisuc_2315	FSU_2864	–	–	Yes	esterase	–	–
CE6	Fisuc_2534	FSU_3103	CBM6	BTD	Yes	acetyl xylan esterase	xylan esterase	This work
CE6	Fisuc_2800	FSU_0054	–	–	Yes	acetyl xylan esterase	–	–
CE8	Fisuc_0679	FSU_1115	CBM35	–	No	pectin esterase	–	–
CE12	Fisuc_1995	FSU_2518	–	–	Yes	esterase	–	–
CE12	Fisuc_2478	FSU_3044	CBM35	–	Yes	esterase	–	–
CE12	Fisuc_2479	FSU_3045	CBM35	–	No	esterase	–	–
CE15	Fisuc_2348	FSU_2898	–	–	No	esterase	–	–

^aFSU locus tags refer to the equivalent ORF call in the F. succinogenes genome sequence project described by GenBank accession: CP002158.

^bBasic Terminal Domain (BTD) is also known as F. succinogenes-specific paralogous module 1 (FPm-1) [46].