Figure 4. N-GlcNAc2 produced by repression of Alg1 cross-reacts with CTD110.6 antibodies.
a, An immunoblot showing CTD110.6 antibody of Alg1-ts-mutant yeast culture medium. b, An immunoblot showing anti-Alg1 antibody reaction over the time course during incubation of T24 cells in glucose deprivation medium (0 mM glucose). An anti-α-tubulin antibody was used as an internal control. c, The effects of treatment with siRNA specific for Alg1 on the expression of proteins that reacted with CTD110.6 antibodies under glucose deprivation. The left panel shows an immunoblot for CTD110.6, anti-ALG1, anti-ORP150 and anti-α-tubulin antibodies. The right panel shows a quantitative analysis of reactivity with the CTD110.6 antibody and with ALG1 in three experiments, all normalized to the anti-α-tubulin signal for untreated samples in high-glucose medium. d, A schematic summary of all of the experimental results and the hypothesis.