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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Autoimmun. 2011 Feb 17;36(3-4):210–220. doi: 10.1016/j.jaut.2011.01.006

Fig. 4. Treg frequency increases after nasal CM peptide immunization.

Fig. 4

(A) Spleen and or blood derived lymphocytes from CM peptide-immunized mice were stained and analyzed with flow cytometry to determine the frequency of Treg (CD25+Foxp3+). The frequency was determined over time after gating on total CD4+/CD127low T cells, n= 4 mice per group. The data are significantly different among CM-peptide treated group and no treatment only for the indicated time points. These can be identified by the asterisk “*”, which determines a p value <0.05. (B) Representative flow cytometry plots indicating the frequency of Treg in the spleen after gating on CD4+/CD127low cells, 35 days post infection (p.i.) with hpCVB3. (C) Treg frequency in the heart infiltrate at the indicated time points p.i. (D) Histological scoring and representative microphotographs from hearts of mice after receiving 1×10^6 CFSE-labeled CD4+CD25+ or CD4+CD25− cells from CM combo peptide immunized mice.