Fig 4.
A flowchart of identification of hERG channel blockers. Eighty-eight compounds with class 1.1, 1.2, and 2.1 curves were identified from primary screening. Nineteen compounds with IC50 values less than 10 μM were selected based on the first run of primary screening for further studies. Seventeen of nineteen compounds were confirmed in thallium influx assay, and further tested in a whole cell patch clamp experiment. Twelve of these compounds were confirmed as hERG channel blockers in a whole cell patch clamp experiment and had no off target activity in the non-hERG transduced cells. Nine of the twelve compounds had either low potency or no activity in the KV1.3 channel inhibition assay.