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. Author manuscript; available in PMC: 2012 Sep 9.

Published in final edited form as: Vaccine. 2011 Jan 1;29(39):6802–6809. doi: 10.1016/j.vaccine.2010.12.062

Fig 2 — Intracellular cytokine staining (ICS) analysis of IFN-γ production in CD8+ and CD4+ T cells isolated from mouse spleens immunized with V.opt DNA vaccine. (A) A set of sample FACS data, showing IFN-γ production in CD8+ or CD4+ T cells with LcrV-7, LcrV-8, or HIV-V3 peptide stimulation, as indicated. (B) Percent of peptide-specific IFN-γ production cells in CD8+ or CD4+ T cells following LcrV-7, LcrV-8, or HIV-V3 peptide stimulation. Data represent the average of 5 mice/group ± standard deviation. “*” shows the statistical difference, p<0.05. Splenocytes were collected at 1 week after the last (4^th) DNA immunization.

Fig 2 — Intracellular cytokine staining (ICS) analysis of IFN-γ production in CD8+ and CD4+ T cells isolated from mouse spleens immunized with V.opt DNA vaccine. (A) A set of sample FACS data, showing IFN-γ production in CD8+ or CD4+ T cells with LcrV-7, LcrV-8, or HIV-V3 peptide stimulation, as indicated. (B) Percent of peptide-specific IFN-γ production cells in CD8+ or CD4+ T cells following LcrV-7, LcrV-8, or HIV-V3 peptide stimulation. Data represent the average of 5 mice/group ± standard deviation. “*” shows the statistical difference, p<0.05. Splenocytes were collected at 1 week after the last (4^th) DNA immunization.