Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2012 Jun 1.

Published in final edited form as: Neurobiol Dis. 2011 Feb 3;42(3):292–299. doi: 10.1016/j.nbd.2011.01.019

Lack of CD36 in hippocampal slices does not confer neuroprotection against OGD. Hippocampal slice cultures were cultured for 14 days and subjected to OGD for 45 min, followed by 23 hrs of normoxic incubation in culture medium (reperfusion). Cell death was assessed based on propidium iodide (PI) uptake. No difference in cell death was observed between CD36 KO and WT slices. (A): representative photomicrographs of hippocampal slices from CD36 KO and WT mice under the indicated treatment conditions. Baseline: PI uptake before OGD. OGD: Slices treated with OGD for 45 min. Max: maximum degree of cell death (PI uptake), observed after overnight incubation with 1mM of NMDA. (B). Quantitative assessment of cell death at 24, 48 and 72 after OGD expressed as % of max PI uptake (see above). *p<0.05 from the corresponding sham group; analysis of variance and Newman-Keuls test; n: 35–50 slices/group, from 7 separate experiments. (C) Effect of Pam3CSK4 on cell death after OGD. Pam3CSK4 did not increase the injury in CD36+/+ or CD36−/− slices (p>0.05; n=18–24, from 3 experiments).

Lack of CD36 in hippocampal slices does not confer neuroprotection against OGD. Hippocampal slice cultures were cultured for 14 days and subjected to OGD for 45 min, followed by 23 hrs of normoxic incubation in culture medium (reperfusion). Cell death was assessed based on propidium iodide (PI) uptake. No difference in cell death was observed between CD36 KO and WT slices. (A): representative photomicrographs of hippocampal slices from CD36 KO and WT mice under the indicated treatment conditions. Baseline: PI uptake before OGD. OGD: Slices treated with OGD for 45 min. Max: maximum degree of cell death (PI uptake), observed after overnight incubation with 1mM of NMDA. (B). Quantitative assessment of cell death at 24, 48 and 72 after OGD expressed as % of max PI uptake (see above). *p<0.05 from the corresponding sham group; analysis of variance and Newman-Keuls test; n: 35–50 slices/group, from 7 separate experiments. (C) Effect of Pam3CSK4 on cell death after OGD. Pam3CSK4 did not increase the injury in CD36+/+ or CD36−/− slices (p>0.05; n=18–24, from 3 experiments).