Fig. 2.

Brk suppression induces chromatin fragmentation and suppression of EGFR. Brk levels were suppressed in normal human primary keratinocytes by RNAi. a 48 h following the second transfection with control (Control) or Brk-targeting siRNA (Brk siRNA), cells were harvested and assayed for chromatin fragmentation. As a positive control, primary keratinocytes were also cultured in methyl cellulose (MC) for 48 h prior to harvesting and assaying with the cell death ELISA. *P = 0.036. b Parallel wells were also lysed in hot SDS-PAGE lysis buffer and relative protein levels determined by western blotting