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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Surgery. 2011 May;149(5):689–698. doi: 10.1016/j.surg.2011.02.007

Figure 2.

Figure 2

NIR sentinel lymph node mapping with the imaging system and indocyanine green. (A) NIR fluorescence image of a mouse at 5 minutes post-injection. Blue arrow indicates the putative sentinel lymph node. Green arrows indicate the injection points. (B) White light image of a mouse at 5 minutes post-injection, acquired under room light illumination. (C) NIR-white light merge image mice at 5 minutes post-injection. NIR fluorescence is pseudo colored in red and superimposed on white light image. The blue arrow indicates the putative sentinel lymph node at an axilllary position. Green arrows indicate the injection points (D) Ex vivo inspection of resected lymph nodes shown in NIR-light merge image. NIR fluorescence is psedocolored in red and superimposed on white light image. A blue arrow indicates the putative sentinel lymph node removed from an axillary position, while a yellow arrow indicates an ischiatic lymph node taken from the hindquarter as non-fluorescent control. (E) Histology of resected SLNs showing a representative image of a frozen section of SLNs with H&E staining.