Figure 2. Survival of Cdc10-less Cells Requires Homodimerization of Cdc10 by Its G Interface.
(A) As in 1C, for cells of the indicated genotype; red asterisk, W364A mutation at G interface of Cdc3. (B) Morphology of representative cells of isogenic CDC3+ (left) or cdc3(W364A) strains (right) (derived from JTY5071) grown at 30°C, examined by differential interference contrast (DIC) microscopy (left panels) and co-expressing Cdc10-GFP (from plasmid pLA10), whose localization was determined by epifluorescence microscopy (right panels). See also Figure S2.