Cultures of the indicated genotype were grown in YPGal, spotted in five-fold serial dilutions onto agar plates containing SCGal-Leu medium in the absence (left) or presence (right) of FOA, and photographed after incubation at RT for 5–6 dy. Far right, schematic depiction of septin composition in cells that have lost the URA3 plasmid; red asterisk, Δα0 mutation at the NC interface of Cdc11; grey spheres, septin subunits of complexes where Cdc11 normally occupies the terminal position; blue spheres, septin subunits of complexes where Shs1 normally occupies the terminal position. See also Figure S4.