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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Dev Cell. 2011 Apr 19;20(4):540–549. doi: 10.1016/j.devcel.2011.02.004

Figure 6. Septin Localization and Barrier Function in Cdc10-less and Cdc11-less Cells.

Figure 6

Cells of the genotype shown, expressing from either CEN plasmids (in brackets) or from the chomosomal locus (for CDC10-mCherry) the indicated fluorescently-tagged proteins, were grown to mid-exponential phase and examined by DIC (top or left) and by epifluorescence microscopy with appropriate filters to visualize either Cdc12-CFP, in presence and absence of Cdc10-mCherry, or Cdc12-GFP, and either Sec3-GFP or Chs2-GFP (bottom panels). Strains were JTY3992 (A), 4003482™ (B), FOA-resistant derivative of JTY4944 (C), and JTY5140 (D and E). Plasmids were pSEC3-GFP3-1, YCpH-CDC12-CFP, YCp-CHS2-iGFP, and pLP17 (CDC12-GFP). Arrowheads, split septin rings; Arrows, bud neck localization of non-septin proteins. See also Figure S5.