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Published in final edited form as: Glia. 2010 Dec 29;59(3):486–498. doi: 10.1002/glia.21118

S100B suppression is mediated through STAT3. (A–C) Glioma-conditioned medium (GCM) and low levels of S100B induce STAT3 expression and phosphorylation in microglia. N9 cells were treated with GCM and different doses of S100B as indicated. Samples were collected at 6 h and analyzed for STAT3 activity by RT-PCR (A), Western blotting (B), and FACS (C). (D) S100B suppression was not seen in STAT3-deficient cells. Bone marrow-derived monocytes from Stat3^−/− and corresponding control mice were incubated with S100B for 24 h prior to IL-1β RT-PCR. Experimental results are representative of two to three separate experiments. (n=3 samples/group, ±SEM)

S100B suppression is mediated through STAT3. (A–C) Glioma-conditioned medium (GCM) and low levels of S100B induce STAT3 expression and phosphorylation in microglia. N9 cells were treated with GCM and different doses of S100B as indicated. Samples were collected at 6 h and analyzed for STAT3 activity by RT-PCR (A), Western blotting (B), and FACS (C). (D) S100B suppression was not seen in STAT3-deficient cells. Bone marrow-derived monocytes from Stat3^−/− and corresponding control mice were incubated with S100B for 24 h prior to IL-1β RT-PCR. Experimental results are representative of two to three separate experiments. (n=3 samples/group, ±SEM)