Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Glia. 2010 Dec 29;59(3):486–498. doi: 10.1002/glia.21118

Figure 7.

Figure 7

Blockage of RAGE inhibits glioma-induced STAT3 activation in N9 microglia (MG). (A) Western blot demonstrating an increase in STAT3 phosphorylation and expression in N9 MG 6 h after incubation with GL261 glioma-conditioned medium (GCM). Anti-RAGE blocking antibody (αRAGE) reversed STAT3 activation. (B) EMSA demonstrating an increase in STAT3 DNA binding following incubation of N9 cells with GCM. αRAGE inhibited glioma-induced STAT3 activation in N9 MG. (C) STAT3 transcription activity was examined by IL-10 RT-PCR 6 h after incubation of N9 cells with GCM. IL-10 expression was inhibited by αRAGE but not IgG. Experimental results are representative of two separate experiments. (n=3 samples/group, ±SEM)