Figure 4.

Phenotype of hGRPs transplanted into focally demyelinated rat spinal cord. (A–C) Three weeks after tranplantation, many of hGRPs co-localized with the Olig2 marker (A). Co-expression of HuNA and GFAP was quite frequent (B). MBP expression at this time was not detected (C). (D–F) Seven weeks after transplantation, 17±4% of hGRPs expressed Olig2 (D), and many were GFAP positive (E). No MBP expression in hGRPs was detected (F). (G–I) Fourteen weeks after grafting, 3.8±3.62% of hGRPs co-expressed Olig2 (G), and a large proportion was GFAP positive (H, arrowheads). Inset in G shows triple immunostaining for GFAP (blue) Olig2 (magenta) and HuNA (green). A rare example of a cell expressing all three markers is shown with the orthogonal view. At this time point, single MBP-expressing human cells were detected within the lesion, which represented 0.68±1% of total hGRPs (I).