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SDS–PAGE analysis of the purified LR11 Vps10p domain before and after Endo H treatment. 2 µg of the purified protein was either treated with 10 U Endo H (+) or left untreated (−) and subjected to SDS–PAGE using 8% gel under reducing conditions followed by staining with Coomassie Brilliant Blue. Lane M contains molecular-weight markers (labelled in kDa).
