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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Glia. 2010 Dec 29;59(3):472–485. doi: 10.1002/glia.21117

Figure 1. GCM activated primary microglia.

Figure 1

Microglia were treated with GCM or DMEM+1% FBS (microglial medium) or 10% FBS (glioma medium) as controls. Cells were observed under bright field (A), or immunofluorescently stained with Iba1 antibody and labeled with DAPI (B), or lysed to obtain protein extracts for western blotting (C). Iba1 expression was normalized to α-tubulin. The experiment was repeated 3 times. Arrows: amoeboid microglia.